Pressure response of heavy meromyosin in solution
نویسندگان
چکیده
منابع مشابه
Solution structure of heavy meromyosin by small-angle scattering.
Elucidation of x-ray crystal structures for the S1 subfragment of myosin afforded atomic resolution of the nucleotide and actin binding sites of the enzyme. The structures have led to more detailed hypotheses regarding the mechanisms by which force generation is coupled to ATP hydrolysis. However, the three-dimensional structure of double-headed myosin consisting of two S1 subfragments has not ...
متن کاملInteraction of Heavy Meromyosin with Substrate I. DIFFERENCE IN ULTRAVIOLET ABSORPTION SPECTRUM BETWEEN HEAVY MEROMYOSIN
Difference spectra induced by ATP and its analogues of the ultraviolet absorption of heavy meromyosin around 280 rnp were measured by the double cell method. The difference spectrum induced by ATP showed two peaks, at 281 and 289 rnp, and a shoulder near 300 mp. The maximum value of the difference molar extinction coefficient, AE, at 289 mp, was 5000 f 300 Me1 cm-l. The difference spectrum caus...
متن کاملThe interaction of actin with myosin and heavy meromyosin in solution at low ionic strength.
The interaction of actin with myosin and heavy meromyosin was studied in the presence of Mgf+ ion and adenosine triphosphate at low ionic strength. When the heavy meromyosin ATPase was 17-fold activated by actin, the actoheavy meromyosin complex responsible for this activation did not give rise to a measurable increase in the viscosity of the sample above that predicted for a noninteracting mix...
متن کاملInteraction of heavy meromyosin with substrate. I. Difference in ultraviolet absorption spectrum between heavy meromyosin and its Michaelis-Menten complex.
Difference spectra induced by ATP and its analogues of the ultraviolet absorption of heavy meromyosin around 280 rnp were measured by the double cell method. The difference spectrum induced by ATP showed two peaks, at 281 and 289 rnp, and a shoulder near 300 mp. The maximum value of the difference molar extinction coefficient, AE, at 289 mp, was 5000 f 300 Me1 cm-l. The difference spectrum caus...
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Consden, R. & Gordon, A. H. (1948). Nature, Lond., 162, 180. Dixon, J. S. & Lipkin, D. (1954). Analyt. Chem. 26, 1092. Elliott, S. D. (1962). Nature, Lond., 193, 1105. Foster, A. B., Horton, D. & Stacey, M. (1957). J. chem. Soc. p. 81. Gelotte, B. (1960). J. Chromat. 3, 330. Hanahan, D. J. & Olley, J. N. (1958). J. biol. Chem. 231, 813. Hanes, C. S. & Isherwood, F. A. (1949). Nature, Lond., 164...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2003
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.43.s126_4